Hotspot

psbE-petL

LSC · 64639-65787 · 1149 bp

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Pi 0.0252
MarkerSeek score 69.70
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1149
variable_sites
73
pi
0.0252
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.94
markerseek_score
69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 AGAACGTTCTCCTGTGCTTC AGTAGAAAACCGAAATAACTAGT 50.0 30.4 57.8 53.2 1177-1211 bp 1.0 48.9
psbE-petL_p2 GTTCTCCTGTGCTTCCAGAC AGTAGAAAACCGAAATAACTAGT 55.0 30.4 58.2 53.2 1172-1206 bp 1.0 48.9
psbE-petL_p3 CAACCCGCAATGAATAGGGA AGTAGAAAACCGAAATAACTAGT 50.0 30.4 57.9 53.2 1254-1288 bp 1.0 48.6
psbE-petL_p4 CAACCCGCAATGAATAGGGA ACCGAAATAACTAGTTATAGTAAGCA 50.0 30.8 57.9 56.1 1246-1280 bp 1.0 48.6
psbE-petL_p5 ACCAACCCGCAATGAATAGG AGTAGAAAACCGAAATAACTAGT 50.0 30.4 58.2 53.2 1256-1290 bp 1.0 48.6