Hotspot

rps16

LSC · 54715-55895 · 1181 bp

Back to Uraria

Pi 0.00345
MarkerSeek score 60.40
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1181
variable_sites
4
pi
0.00345
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 ACTGGATCCAAGCTAAAGGA AGATAACAAGAGAGCGGGGG 45.0 55.0 55.8 58.9 1272-1294 bp 1.0 57.3
rps16_p2 ACTGGATCCAAGCTAAAGGA TATAAGTCATCCGCACCCCC 45.0 55.0 55.8 58.9 1219-1241 bp 1.0 56.8
rps16_p3 ACTGGATCCAAGCTAAAGGA AAGATAACAAGAGAGCGGGG 45.0 50.0 55.8 57.0 1273-1295 bp 1.0 56.3
rps16_p4 ACTGGATCCAAGCTAAAGGAA AGATAACAAGAGAGCGGGGG 42.9 55.0 56.6 58.9 1272-1294 bp 1.0 55.6
rps16_p5 ACTGGATCCAAGCTAAAGGAA TATAAGTCATCCGCACCCCC 42.9 55.0 56.6 58.9 1219-1241 bp 1.0 55.1