Hotspot

rps3-rps19

LSC · 82223-82673 · 451 bp

Back to Uraria

Pi 0.00701
MarkerSeek score 64.40
Primer available yes
Label rps3-rps19

Feature Metadata

label_name
rps3-rps19
feature_type
igs
region
LSC
length_bp
451
variable_sites
3
pi
0.00701
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3-rps19_p1 CAAGTCTGAAACCAAGCGGA TAAACTTCCGAGGACATGCG 50.0 50.0 58.4 58.0 528-535 bp 1.0 80.6
rps3-rps19_p2 CAAGTCTGAAACCAAGCGGA TCCGAGGACATGCGAAAAAT 50.0 45.0 58.4 57.9 522-529 bp 1.0 79.8
rps3-rps19_p3 CAAGTCTGAAACCAAGCGGA TTCCGAGGACATGCGAAAAA 50.0 45.0 58.4 58.1 523-530 bp 1.0 79.8
rps3-rps19_p4 AAGTCTGAAACCAAGCGGAT TAAACTTCCGAGGACATGCG 45.0 50.0 57.4 58.0 527-534 bp 1.0 79.3
rps3-rps19_p5 AGTCTGAAACCAAGCGGATT TAAACTTCCGAGGACATGCG 45.0 50.0 57.4 58.0 526-533 bp 1.0 79.3