Hotspot

petA-psbJ

LSC · 61100-61986 · 887 bp

Back to Uraria

Pi 0.00338
MarkerSeek score 60.70
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
887
variable_sites
3
pi
0.00338
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CGTGTCCAAGGTCTCTTGTT CATATTCCGGATTGGGCTCA 50.0 50.0 57.8 57.4 1027-1031 bp 1.0 73.8
petA-psbJ_p2 CGTTACGTGTCCAAGGTCTC CATATTCCGGATTGGGCTCA 55.0 50.0 58.3 57.4 1032-1036 bp 1.0 73.3
petA-psbJ_p3 GATCCGTTACGTGTCCAAGG CATATTCCGGATTGGGCTCA 55.0 50.0 58.4 57.4 1036-1040 bp 1.0 72.8
petA-psbJ_p4 CGTGTCCAAGGTCTCTTGTT TATTCCGGATTGGGCTCATC 50.0 50.0 57.8 57.1 1025-1029 bp 1.0 71.4
petA-psbJ_p5 CGTGTCCAAGGTCTCTTGTT TCATATTCCGGATTGGGCTC 50.0 50.0 57.8 57.1 1028-1032 bp 1.0 71.4