Hotspot

psaI-ycf4

LSC · 58352-58613 · 262 bp

Back to Uraria

Pi 0.0158
MarkerSeek score 56.90
Primer available yes
Label psaI-ycf4

Feature Metadata

label_name
psaI-ycf4
feature_type
igs
region
LSC
length_bp
262
variable_sites
4
pi
0.0158
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaI-ycf4_p1 ATTTCCGGCAATTGCAATGG CAAAAAGACACCCGCGATTC 45.0 50.0 58.0 58.0 392-401 bp 1.0 82.1
psaI-ycf4_p2 GGCAATTGCAATGGCTTCTT CAAAAAGACACCCGCGATTC 45.0 50.0 57.9 58.0 386-395 bp 1.0 81.5
psaI-ycf4_p3 ATTTCCGGCAATTGCAATGG ATATAAACCCAGCACGAGCC 45.0 50.0 58.0 57.7 371-380 bp 1.0 80.5
psaI-ycf4_p4 GGCAATTGCAATGGCTTCTT ATATAAACCCAGCACGAGCC 45.0 50.0 57.9 57.7 365-374 bp 1.0 79.9
psaI-ycf4_p5 ATTTCCGGCAATTGCAATGG TCAAAAAGACACCCGCGATT 45.0 45.0 58.0 58.4 393-402 bp 1.0 79.2