Hotspot

atpH-atpF

LSC · 47845-48490 · 646 bp

Back to Uraria

Pi 0.00637
MarkerSeek score 65.10
Primer available yes
Label atpH-atpF

Feature Metadata

label_name
atpH-atpF
feature_type
igs
region
LSC
length_bp
646
variable_sites
4
pi
0.00637
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpF_p1 GGCTGGTTGTAGCATTAGCA CCAGTAGCCCAAACAAAGGA 50.0 50.0 58.2 57.7 735 bp 1.0 78.1
atpH-atpF_p2 TGGGCTGGTTGTAGCATTAG CCAGTAGCCCAAACAAAGGA 50.0 50.0 57.6 57.7 737 bp 1.0 76.8
atpH-atpF_p3 AGGCAGAGGGAAAAATACGG CCAGTAGCCCAAACAAAGGA 50.0 50.0 57.6 57.7 804 bp 1.0 76.5
atpH-atpF_p4 CCAGAGGCAGAGGGAAAAAT CCAGTAGCCCAAACAAAGGA 50.0 50.0 57.5 57.7 808 bp 1.0 75.7
atpH-atpF_p5 GCAGAGGGAAAAATACGGGG CCAGTAGCCCAAACAAAGGA 55.0 50.0 58.6 57.7 802 bp 1.0 75.0