Hotspot

psbM-petN

LSC · 31925-32652 · 728 bp

Back to Uraria

Pi 0.0124
MarkerSeek score 65.90
Primer available yes
Label psbM-petN

Feature Metadata

label_name
psbM-petN
feature_type
igs
region
LSC
length_bp
728
variable_sites
9
pi
0.0124
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-petN_p1 TGCTACTGCACTGTTTATTCT TCTTGCTTGGGCTGCTTTAA 38.1 45.0 55.2 58.0 883-913 bp 1.0 50.7
psbM-petN_p2 TGCTACTGCACTGTTTATTCT TTTTTCCCTTTCCCTCGTGG 38.1 50.0 55.2 58.0 850-880 bp 1.0 50.7
psbM-petN_p3 TGCTACTGCACTGTTTATTCT CGTGGTATGGGGAAGAAGTG 38.1 55.0 55.2 58.0 835-865 bp 1.0 50.6
psbM-petN_p4 TGCTACTGCACTGTTTATTCT TTCCCTTTCCCTCGTGGTAT 38.1 50.0 55.2 58.0 847-877 bp 1.0 50.5
psbM-petN_p5 TGCTACTGCACTGTTTATTCT TCGTGGTATGGGGAAGAAGT 38.1 50.0 55.2 58.1 836-866 bp 1.0 50.4