Hotspot

trnS-UGA-psbC

LSC · 25917-26153 · 237 bp

Back to Uraria

Pi 0.0759
MarkerSeek score 82.80
Primer available yes
Label trnS-UGA-psbC

Feature Metadata

label_name
trnS-UGA-psbC
feature_type
igs
region
LSC
length_bp
237
variable_sites
18
pi
0.0759
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-UGA-psbC_p1 GAGAGATGGCTGAGTGGTTG TTTATGGCACGCAGGAAGAG 55.0 50.0 58.0 58.3 429 bp 1.0 81.1
trnS-UGA-psbC_p2 GCTGAGTGGTTGATAGCTCC TTTATGGCACGCAGGAAGAG 55.0 50.0 58.1 58.3 421 bp 1.0 80.9
trnS-UGA-psbC_p3 GGCTGAGTGGTTGATAGCTC TTTATGGCACGCAGGAAGAG 55.0 50.0 58.1 58.3 422 bp 1.0 80.9
trnS-UGA-psbC_p4 GGTTGATAGCTCCGGTCTTG TTTATGGCACGCAGGAAGAG 55.0 50.0 58.1 58.3 414 bp 1.0 80.9
trnS-UGA-psbC_p5 GCTTTTGGAGAGATGGCTGA TTTATGGCACGCAGGAAGAG 50.0 50.0 57.9 58.3 436 bp 1.0 80.2