Hotspot

trnM-CAU-ndhC

LSC · 9518-10639 · 1122 bp

Back to Uraria

Pi 0.00359
MarkerSeek score 61.40
Primer available yes
Label trnM-CAU-ndhC

Feature Metadata

label_name
trnM-CAU-ndhC
feature_type
igs
region
LSC
length_bp
1122
variable_sites
4
pi
0.00359
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-ndhC_p1 CCCGCCGTATGAAAGCAATA CAGGTCCTTTGCTAATCGGG 50.0 55.0 58.4 58.3 1281-1307 bp 1.0 74.7
trnM-CAU-ndhC_p2 CCCGCCGTATGAAAGCAATA CCTTTGCTAATCGGGGCTAA 50.0 50.0 58.4 57.7 1276-1302 bp 1.0 74.7
trnM-CAU-ndhC_p3 CCCGCCGTATGAAAGCAATA TCCTTTGCTAATCGGGGCTA 50.0 50.0 58.4 58.5 1277-1303 bp 1.0 73.4
trnM-CAU-ndhC_p4 CCGCCGTATGAAAGCAATAC CAGGTCCTTTGCTAATCGGG 50.0 55.0 57.3 58.3 1280-1306 bp 1.0 72.7
trnM-CAU-ndhC_p5 CCGCCGTATGAAAGCAATAC CCTTTGCTAATCGGGGCTAA 50.0 50.0 57.3 57.7 1275-1301 bp 1.0 72.6