Hotspot

rbcL-atpB

LSC · 6645-7406 · 762 bp

Back to Uraria

Pi 0.00263
MarkerSeek score 60.40
Primer available yes
Label rbcL-atpB

Feature Metadata

label_name
rbcL-atpB
feature_type
igs
region
LSC
length_bp
762
variable_sites
2
pi
0.00263
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-atpB_p1 AACACCAGCTTTGAACCCAA CTACATCTAGTACCGGGCCA 45.0 55.0 58.1 58.0 913-937 bp 1.0 79.1
rbcL-atpB_p2 AACACCAGCTTTGAACCCAA GTTTCAAGCGCAGAAACCTC 45.0 50.0 58.1 58.0 863-887 bp 1.0 78.8
rbcL-atpB_p3 AACACCAGCTTTGAACCCAA CCTGGGGGAAAAGCTACATC 45.0 55.0 58.1 57.9 926-950 bp 1.0 78.7
rbcL-atpB_p4 AACACCAGCTTTGAACCCAA ACATCTAGTACCGGGCCAAT 45.0 50.0 58.1 58.2 911-935 bp 1.0 77.6
rbcL-atpB_p5 GCTTTGAACCCAACACTTGC CTACATCTAGTACCGGGCCA 50.0 55.0 58.4 58.0 906-930 bp 1.0 76.8