Hotspot

matK-rbcL

LSC · 3579-5216 · 1638 bp

Back to Uraria

Pi 0.00373
MarkerSeek score 62.30
Primer available yes
Label matK-rbcL

Feature Metadata

label_name
matK-rbcL
feature_type
igs
region
LSC
length_bp
1638
variable_sites
6
pi
0.00373
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-rbcL_p1 CCACGACTATAAGCGAGTCC AGTCCTGAATTAGCTGCTGC 55.0 50.0 57.6 58.0 1793-1820 bp 1.0 78.0
matK-rbcL_p2 CCACGACTATAAGCGAGTCC CTGCCAAATGGAGTCCTGAA 55.0 50.0 57.6 57.8 1804-1831 bp 1.0 76.6
matK-rbcL_p3 GGTGGCGAGATCTATGGAGT AGTCCTGAATTAGCTGCTGC 55.0 50.0 58.7 58.0 1732-1759 bp 1.0 75.9
matK-rbcL_p4 CCACGACTATAAGCGAGTCC TGGAAGGCGATCAAATTCGA 55.0 45.0 57.6 57.6 1763-1790 bp 1.0 75.2
matK-rbcL_p5 GGTGGCGAGATCTATGGAGT CTGCCAAATGGAGTCCTGAA 55.0 50.0 58.7 57.8 1743-1770 bp 1.0 74.6