primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK-rbcL_p1	matK-rbcL	1	CCACGACTATAAGCGAGTCC	AGTCCTGAATTAGCTGCTGC	20	20	55.0	50.0	57.6	58.0	0.0	13.4	0.438170	3579	5216	1793-1820	1.000000	78.0
matK-rbcL_p2	matK-rbcL	2	CCACGACTATAAGCGAGTCC	CTGCCAAATGGAGTCCTGAA	20	20	55.0	50.0	57.6	57.8	0.0	11.3	0.607662	3579	5216	1804-1831	1.000000	76.6
matK-rbcL_p3	matK-rbcL	3	GGTGGCGAGATCTATGGAGT	AGTCCTGAATTAGCTGCTGC	20	20	55.0	50.0	58.7	58.0	0.0	13.4	0.702353	3579	5216	1732-1759	1.000000	75.9
matK-rbcL_p4	matK-rbcL	4	CCACGACTATAAGCGAGTCC	TGGAAGGCGATCAAATTCGA	20	20	55.0	45.0	57.6	57.6	0.0	0.0	0.797569	3579	5216	1763-1790	1.000000	75.2
matK-rbcL_p5	matK-rbcL	5	GGTGGCGAGATCTATGGAGT	CTGCCAAATGGAGTCCTGAA	20	20	55.0	50.0	58.7	57.8	0.0	11.3	0.871846	3579	5216	1743-1770	1.000000	74.6
rbcL-atpB_p1	rbcL-atpB	1	AACACCAGCTTTGAACCCAA	CTACATCTAGTACCGGGCCA	20	20	45.0	55.0	58.1	58.0	0.0	6.3	0.149577	6645	7406	913-937	1.000000	79.1
rbcL-atpB_p2	rbcL-atpB	2	AACACCAGCTTTGAACCCAA	GTTTCAAGCGCAGAAACCTC	20	20	45.0	50.0	58.1	58.0	0.0	31.5	0.178639	6645	7406	863-887	1.000000	78.8
rbcL-atpB_p3	rbcL-atpB	3	AACACCAGCTTTGAACCCAA	CCTGGGGGAAAAGCTACATC	20	20	45.0	55.0	58.1	57.9	0.0	0.0	0.199291	6645	7406	926-950	1.000000	78.7
rbcL-atpB_p4	rbcL-atpB	4	AACACCAGCTTTGAACCCAA	ACATCTAGTACCGGGCCAAT	20	20	45.0	50.0	58.1	58.2	0.0	6.3	0.338145	6645	7406	911-935	1.000000	77.6
rbcL-atpB_p5	rbcL-atpB	5	GCTTTGAACCCAACACTTGC	CTACATCTAGTACCGGGCCA	20	20	50.0	55.0	58.4	58.0	0.0	6.3	0.438066	6645	7406	906-930	1.000000	76.8
trnM-CAU-ndhC_p1	trnM-CAU-ndhC	1	CCCGCCGTATGAAAGCAATA	CAGGTCCTTTGCTAATCGGG	20	20	50.0	55.0	58.4	58.3	0.0	0.0	0.742907	9518	10639	1281-1307	1.000000	74.7
trnM-CAU-ndhC_p2	trnM-CAU-ndhC	2	CCCGCCGTATGAAAGCAATA	CCTTTGCTAATCGGGGCTAA	20	20	50.0	50.0	58.4	57.7	0.0	0.0	0.753172	9518	10639	1276-1302	1.000000	74.7
trnM-CAU-ndhC_p3	trnM-CAU-ndhC	3	CCCGCCGTATGAAAGCAATA	TCCTTTGCTAATCGGGGCTA	20	20	50.0	50.0	58.4	58.5	0.0	0.0	0.908636	9518	10639	1277-1303	1.000000	73.4
trnM-CAU-ndhC_p4	trnM-CAU-ndhC	4	CCGCCGTATGAAAGCAATAC	CAGGTCCTTTGCTAATCGGG	20	20	50.0	55.0	57.3	58.3	0.0	0.0	0.994860	9518	10639	1280-1306	1.000000	72.7
trnM-CAU-ndhC_p5	trnM-CAU-ndhC	5	CCGCCGTATGAAAGCAATAC	CCTTTGCTAATCGGGGCTAA	20	20	50.0	50.0	57.3	57.7	0.0	0.0	1.005125	9518	10639	1275-1301	1.000000	72.6
trnS-UGA-psbC_p1	trnS-UGA-psbC	1	GAGAGATGGCTGAGTGGTTG	TTTATGGCACGCAGGAAGAG	20	20	55.0	50.0	58.0	58.3	0.0	0.0	0.283517	25917	26153	429-429	1.000000	81.1
trnS-UGA-psbC_p2	trnS-UGA-psbC	2	GCTGAGTGGTTGATAGCTCC	TTTATGGCACGCAGGAAGAG	20	20	55.0	50.0	58.1	58.3	0.0	0.0	0.315417	25917	26153	421-421	1.000000	80.9
trnS-UGA-psbC_p3	trnS-UGA-psbC	3	GGCTGAGTGGTTGATAGCTC	TTTATGGCACGCAGGAAGAG	20	20	55.0	50.0	58.1	58.3	0.0	0.0	0.315417	25917	26153	422-422	1.000000	80.9
trnS-UGA-psbC_p4	trnS-UGA-psbC	4	GGTTGATAGCTCCGGTCTTG	TTTATGGCACGCAGGAAGAG	20	20	55.0	50.0	58.1	58.3	0.0	0.0	0.320837	25917	26153	414-414	1.000000	80.9
trnS-UGA-psbC_p5	trnS-UGA-psbC	5	GCTTTTGGAGAGATGGCTGA	TTTATGGCACGCAGGAAGAG	20	20	50.0	50.0	57.9	58.3	0.0	0.0	0.381963	25917	26153	436-436	1.000000	80.2
psbM-petN_p1	psbM-petN	1	TGCTACTGCACTGTTTATTCT	TCTTGCTTGGGCTGCTTTAA	21	20	38.1	45.0	55.2	58.0	0.0	0.0	3.763182	31925	32652	883-913	1.000000	50.7
psbM-petN_p2	psbM-petN	2	TGCTACTGCACTGTTTATTCT	TTTTTCCCTTTCCCTCGTGG	21	20	38.1	50.0	55.2	58.0	0.0	0.0	3.767432	31925	32652	850-880	1.000000	50.7
psbM-petN_p3	psbM-petN	3	TGCTACTGCACTGTTTATTCT	CGTGGTATGGGGAAGAAGTG	21	20	38.1	55.0	55.2	58.0	0.0	0.0	3.781314	31925	32652	835-865	1.000000	50.6
psbM-petN_p4	psbM-petN	4	TGCTACTGCACTGTTTATTCT	TTCCCTTTCCCTCGTGGTAT	21	20	38.1	50.0	55.2	58.0	0.0	2.3	3.796005	31925	32652	847-877	1.000000	50.5
psbM-petN_p5	psbM-petN	5	TGCTACTGCACTGTTTATTCT	TCGTGGTATGGGGAAGAAGT	21	20	38.1	50.0	55.2	58.1	0.0	0.0	3.810154	31925	32652	836-866	1.000000	50.4
atpH-atpF_p1	atpH-atpF	1	GGCTGGTTGTAGCATTAGCA	CCAGTAGCCCAAACAAAGGA	20	20	50.0	50.0	58.2	57.7	0.0	0.0	0.536172	47845	48490	735-735	1.000000	78.1
atpH-atpF_p2	atpH-atpF	2	TGGGCTGGTTGTAGCATTAG	CCAGTAGCCCAAACAAAGGA	20	20	50.0	50.0	57.6	57.7	0.0	0.0	0.704733	47845	48490	737-737	1.000000	76.8
atpH-atpF_p3	atpH-atpF	3	AGGCAGAGGGAAAAATACGG	CCAGTAGCCCAAACAAAGGA	20	20	50.0	50.0	57.6	57.7	0.0	0.0	0.706423	47845	48490	804-804	1.000000	76.5
atpH-atpF_p4	atpH-atpF	4	CCAGAGGCAGAGGGAAAAAT	CCAGTAGCCCAAACAAAGGA	20	20	50.0	50.0	57.5	57.7	0.0	0.0	0.807911	47845	48490	808-808	1.000000	75.7
atpH-atpF_p5	atpH-atpF	5	GCAGAGGGAAAAATACGGGG	CCAGTAGCCCAAACAAAGGA	20	20	55.0	50.0	58.6	57.7	0.0	0.0	0.896809	47845	48490	802-802	1.000000	75.0
trnR-UCU-trnS-GCU_p1	trnR-UCU-trnS-GCU	1	AGAAGACCTCTGTCCTATCCA	CAATCCGACGCTTTAGTCCA	21	20	47.6	50.0	56.9	58.0	0.0	0.0	2.056187	51618	52919	1361-1373	1.000000	64.9
trnR-UCU-trnS-GCU_p2	trnR-UCU-trnS-GCU	2	AGAAGACCTCTGTCCTATCCA	AATCCGACGCTTTAGTCCAC	21	20	47.6	50.0	56.9	58.0	0.0	0.0	2.057575	51618	52919	1360-1372	1.000000	64.9
trnR-UCU-trnS-GCU_p3	trnR-UCU-trnS-GCU	3	AGGTTTAGAAGACCTCTGTCCT	CAATCCGACGCTTTAGTCCA	22	20	45.5	50.0	57.9	58.0	0.0	0.0	2.139515	51618	52919	1367-1379	1.000000	64.2
trnR-UCU-trnS-GCU_p4	trnR-UCU-trnS-GCU	4	AGGTTTAGAAGACCTCTGTCCT	AATCCGACGCTTTAGTCCAC	22	20	45.5	50.0	57.9	58.0	0.0	0.0	2.140903	51618	52919	1366-1378	1.000000	64.2
trnR-UCU-trnS-GCU_p5	trnR-UCU-trnS-GCU	5	AGAAGACCTCTGTCCTATCCA	ACGGAAAGAGAGGGATTCGA	21	20	47.6	50.0	56.9	58.2	0.0	0.0	2.202953	51618	52919	1414-1426	1.000000	63.7
rps16_p1	rps16	1	ACTGGATCCAAGCTAAAGGA	AGATAACAAGAGAGCGGGGG	20	20	45.0	55.0	55.8	58.9	17.0	0.0	3.053931	54715	55895	1272-1294	1.000000	57.3
rps16_p2	rps16	2	ACTGGATCCAAGCTAAAGGA	TATAAGTCATCCGCACCCCC	20	20	45.0	55.0	55.8	58.9	17.0	0.0	3.124435	54715	55895	1219-1241	1.000000	56.8
rps16_p3	rps16	3	ACTGGATCCAAGCTAAAGGA	AAGATAACAAGAGAGCGGGG	20	20	45.0	50.0	55.8	57.0	17.0	0.0	3.167925	54715	55895	1273-1295	1.000000	56.3
rps16_p4	rps16	4	ACTGGATCCAAGCTAAAGGAA	AGATAACAAGAGAGCGGGGG	21	20	42.9	55.0	56.6	58.9	17.0	0.0	3.257266	54715	55895	1272-1294	1.000000	55.6
rps16_p5	rps16	5	ACTGGATCCAAGCTAAAGGAA	TATAAGTCATCCGCACCCCC	21	20	42.9	55.0	56.6	58.9	17.0	0.0	3.327770	54715	55895	1219-1241	1.000000	55.1
psaI-ycf4_p1	psaI-ycf4	1	ATTTCCGGCAATTGCAATGG	CAAAAAGACACCCGCGATTC	20	20	45.0	50.0	58.0	58.0	30.3	8.2	0.063256	58352	58613	392-401	1.000000	82.1
psaI-ycf4_p2	psaI-ycf4	2	GGCAATTGCAATGGCTTCTT	CAAAAAGACACCCGCGATTC	20	20	45.0	50.0	57.9	58.0	32.4	8.2	0.139790	58352	58613	386-395	1.000000	81.5
psaI-ycf4_p3	psaI-ycf4	3	ATTTCCGGCAATTGCAATGG	ATATAAACCCAGCACGAGCC	20	20	45.0	50.0	58.0	57.7	30.3	0.0	0.283121	58352	58613	371-380	1.000000	80.5
psaI-ycf4_p4	psaI-ycf4	4	GGCAATTGCAATGGCTTCTT	ATATAAACCCAGCACGAGCC	20	20	45.0	50.0	57.9	57.7	32.4	0.0	0.359654	58352	58613	365-374	1.000000	79.9
psaI-ycf4_p5	psaI-ycf4	5	ATTTCCGGCAATTGCAATGG	TCAAAAAGACACCCGCGATT	20	20	45.0	45.0	58.0	58.4	30.3	8.2	0.426980	58352	58613	393-402	1.000000	79.2
petA-psbJ_p1	petA-psbJ	1	CGTGTCCAAGGTCTCTTGTT	CATATTCCGGATTGGGCTCA	20	20	50.0	50.0	57.8	57.4	5.6	4.1	0.807326	61100	61986	1027-1031	1.000000	73.8
petA-psbJ_p2	petA-psbJ	2	CGTTACGTGTCCAAGGTCTC	CATATTCCGGATTGGGCTCA	20	20	55.0	50.0	58.3	57.4	0.0	4.1	0.872194	61100	61986	1032-1036	1.000000	73.3
petA-psbJ_p3	petA-psbJ	3	GATCCGTTACGTGTCCAAGG	CATATTCCGGATTGGGCTCA	20	20	55.0	50.0	58.4	57.4	3.5	4.1	0.929845	61100	61986	1036-1040	1.000000	72.8
petA-psbJ_p4	petA-psbJ	4	CGTGTCCAAGGTCTCTTGTT	TATTCCGGATTGGGCTCATC	20	20	50.0	50.0	57.8	57.1	5.6	4.1	1.099659	61100	61986	1025-1029	1.000000	71.4
petA-psbJ_p5	petA-psbJ	5	CGTGTCCAAGGTCTCTTGTT	TCATATTCCGGATTGGGCTC	20	20	50.0	50.0	57.8	57.1	5.6	4.1	1.099659	61100	61986	1028-1032	1.000000	71.4
psbE-petL_p1	psbE-petL	1	AGAACGTTCTCCTGTGCTTC	GACCAATAAATAGAGCGGAGGT	20	22	50.0	45.5	57.8	57.7	24.9	0.0	2.494722	62784	64116	1419-1433	1.000000	61.6
psbE-petL_p2	psbE-petL	2	GTTCTCCTGTGCTTCCAGAC	GACCAATAAATAGAGCGGAGGT	20	22	55.0	45.5	58.2	57.7	0.9	0.0	2.536310	62784	64116	1414-1428	1.000000	61.2
psbE-petL_p3	psbE-petL	3	CCTGCAATGAATAGGGAGGG	GACCAATAAATAGAGCGGAGGT	20	22	55.0	45.5	57.7	57.7	0.0	0.0	2.621525	62784	64116	1493-1507	1.000000	60.5
psbE-petL_p4	psbE-petL	4	AGAACGTTCTCCTGTGCTTC	ACCAATAAATAGAGCGGAGGT	20	21	50.0	42.9	57.8	56.5	24.9	0.0	2.653272	62784	64116	1418-1432	1.000000	60.3
psbE-petL_p5	psbE-petL	5	TCTCCTGTGCTTCCAGACAT	GACCAATAAATAGAGCGGAGGT	20	22	50.0	45.5	58.4	57.7	0.9	0.0	2.689058	62784	64116	1412-1426	1.000000	60.0
psaJ_p1	psaJ	1	CGAACTGAACTAAGAGCGCT	TGGAAGGGTAACACACAAGC	20	20	50.0	50.0	58.0	58.0	11.3	0.0	0.040368	65228	65362	1389-1411	1.000000	81.4
psaJ_p2	psaJ	2	CGAACTGAACTAAGAGCGCT	TTTACCCTTGGCCATGAACC	20	20	50.0	50.0	58.0	58.1	11.3	19.8	0.084392	65228	65362	1171-1193	1.000000	81.4
psaJ_p3	psaJ	3	ACGTTCTGTACATCACGCTC	TGGAAGGGTAACACACAAGC	20	20	50.0	50.0	58.0	58.0	21.0	0.0	0.045799	65228	65362	1457-1479	1.000000	81.3
psaJ_p4	psaJ	4	CGAACTGAACTAAGAGCGCT	CTTGGCCATGAACCTCCTTT	20	20	50.0	50.0	58.0	58.1	11.3	13.3	0.087159	65228	65362	1165-1187	1.000000	81.3
psaJ_p5	psaJ	5	GCGTTCTACCGAACTGAACT	TGGAAGGGTAACACACAAGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.086080	65228	65362	1398-1420	1.000000	81.0
psaJ-rpl33_p1	psaJ-rpl33	1	CGAACTGAACTAAGAGCGCT	TGGAAGGGTAACACACAAGC	20	20	50.0	50.0	58.0	58.0	11.3	0.0	0.040368	65363	65860	1389-1411	1.000000	81.4
psaJ-rpl33_p2	psaJ-rpl33	2	ACGTTCTGTACATCACGCTC	TGGAAGGGTAACACACAAGC	20	20	50.0	50.0	58.0	58.0	21.0	0.0	0.045799	65363	65860	1457-1479	1.000000	81.3
psaJ-rpl33_p3	psaJ-rpl33	3	ACATATCTTTCCGTGGCACC	TGGAAGGGTAACACACAAGC	20	20	50.0	50.0	58.0	58.0	12.9	0.0	0.062727	65363	65860	843-851	1.000000	81.0
psaJ-rpl33_p4	psaJ-rpl33	4	GCGTTCTACCGAACTGAACT	TGGAAGGGTAACACACAAGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.086080	65363	65860	1398-1420	1.000000	81.0
psaJ-rpl33_p5	psaJ-rpl33	5	CGAACTGAACTAAGAGCGCT	AGGGTAACACACAAGCGATC	20	20	50.0	50.0	58.0	57.9	11.3	0.0	0.110790	65363	65860	1385-1407	1.000000	80.8
rpl16-rps3_p1	rpl16-rps3	1	CTCAAGTGCCTGAAGAGCAT	TCCTCTACAAACCATTCGAGC	20	21	50.0	47.6	57.9	57.8	0.0	0.0	1.357907	80248	81565	1535-1541	1.000000	70.0
rpl16-rps3_p2	rpl16-rps3	2	CTCAAGTGCCTGAAGAGCAT	CCTCTACAAACCATTCGAGCT	20	21	50.0	47.6	57.9	57.7	0.0	0.0	1.363875	80248	81565	1534-1540	1.000000	69.9
rpl16-rps3_p3	rpl16-rps3	3	CTCAAGTGCCTGAAGAGCAT	GGTAGGGTTCCTCTACAAACC	20	21	50.0	52.4	57.9	57.4	0.0	14.6	1.734808	80248	81565	1543-1549	1.000000	66.9
rpl16-rps3_p4	rpl16-rps3	4	CTCAAGTGCCTGAAGAGCAT	AGAGAAGGTAGGGTTCCTCT	20	20	50.0	50.0	57.9	56.4	0.0	0.0	1.747947	80248	81565	1549-1555	1.000000	66.8
rpl16-rps3_p5	rpl16-rps3	5	CTCAAGTGCCTGAAGAGCAT	CCTCTACAAACCATTCGAGC	20	20	50.0	50.0	57.9	56.2	0.0	0.0	1.870688	80248	81565	1534-1540	1.000000	65.9
rps3-rps19_p1	rps3-rps19	1	CAAGTCTGAAACCAAGCGGA	TAAACTTCCGAGGACATGCG	20	20	50.0	50.0	58.4	58.0	0.0	0.0	0.411386	82223	82673	528-535	1.000000	80.6
rps3-rps19_p2	rps3-rps19	2	CAAGTCTGAAACCAAGCGGA	TCCGAGGACATGCGAAAAAT	20	20	50.0	45.0	58.4	57.9	0.0	0.0	0.514754	82223	82673	522-529	1.000000	79.8
rps3-rps19_p3	rps3-rps19	3	CAAGTCTGAAACCAAGCGGA	TTCCGAGGACATGCGAAAAA	20	20	50.0	45.0	58.4	58.1	0.0	0.0	0.521244	82223	82673	523-530	1.000000	79.8
rps3-rps19_p4	rps3-rps19	4	AAGTCTGAAACCAAGCGGAT	TAAACTTCCGAGGACATGCG	20	20	45.0	50.0	57.4	58.0	0.0	0.0	0.573205	82223	82673	527-534	1.000000	79.3
rps3-rps19_p5	rps3-rps19	5	AGTCTGAAACCAAGCGGATT	TAAACTTCCGAGGACATGCG	20	20	45.0	50.0	57.4	58.0	0.0	0.0	0.573205	82223	82673	526-533	1.000000	79.3
ndhF-rpl32_p1	ndhF-rpl32	1	CAGATTTTTGGTTGCCGTCG	TGAAAAAGCTTTCAATCTTGTCCA	20	24	50.0	33.3	58.3	57.8	0.0	13.1	4.534114	109277	109905	833-836	1.000000	44.0
ndhF-rpl32_p2	ndhF-rpl32	2	CACACGACGCAGATTTTTGG	TGAAAAAGCTTTCAATCTTGTCCA	20	24	50.0	33.3	58.3	57.8	0.0	13.1	4.545656	109277	109905	842-845	1.000000	43.9
ndhF-rpl32_p3	ndhF-rpl32	3	ACACACGACGCAGATTTTTG	TGAAAAAGCTTTCAATCTTGTCCA	20	24	45.0	33.3	57.6	57.8	0.0	13.1	4.602714	109277	109905	843-846	1.000000	43.5
ndhF-rpl32_p4	ndhF-rpl32	4	CACACACGACGCAGATTTTT	TGAAAAAGCTTTCAATCTTGTCCA	20	24	45.0	33.3	57.6	57.8	0.0	13.1	4.602714	109277	109905	844-847	1.000000	43.5
ndhF-rpl32_p5	ndhF-rpl32	5	ACGACGCAGATTTTTGGTTG	TGAAAAAGCTTTCAATCTTGTCCA	20	24	45.0	33.3	57.6	57.8	0.0	13.1	4.618813	109277	109905	839-842	1.000000	43.3
rps15-trnN-GUU_p1	rps15-trnN-GUU	1	TCAGGAGACAATAGACTTTGACA	TCGATATCTATGGACCATCGA	23	21	39.1	42.9	56.9	55.3	4.9	11.2	7.774557	121267	121386	303-303	1.000000	41.1
rps15-trnN-GUU_p2	rps15-trnN-GUU	2	TCAGGAGACAATAGACTTTGACA	TCGATATCTATGGACCATCGAA	23	22	39.1	40.9	56.9	56.1	4.9	19.1	8.001542	121267	121386	303-303	1.000000	41.1
rps15-trnN-GUU_p3	rps15-trnN-GUU	3	CAGGAGACAATAGACTTTGACA	TCGATATCTATGGACCATCGA	22	21	40.9	42.9	55.5	55.3	4.9	11.2	8.211948	121267	121386	302-302	1.000000	41.1
rps15-trnN-GUU_p4	rps15-trnN-GUU	4	TCAGGAGACAATAGACTTTGACA	ATCGATATCTATGGACCATCGA	23	22	39.1	40.9	56.9	55.8	4.9	21.8	8.215208	121267	121386	304-304	1.000000	41.1
rps15-trnN-GUU_p5	rps15-trnN-GUU	5	TCAGGAGACAATAGACTTTGACA	TCGATATCTATGGACCATCGAAA	23	23	39.1	39.1	56.9	56.8	4.9	19.1	8.295556	121267	121386	303-303	1.000000	41.1