Hotspot

ycf1

SSC · 124984-130608 · 5625 bp

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Pi 0.0105

MarkerSeek score 43.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5625
variable_sites: 170
pi: 0.0105
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACGTCTTTGAGAGCGCATAG	TTGAAAGGTACGTATCGCGG	50.0	50.0	58.1	58.1	657-666 bp	1.0	83.3
ycf1_p2	ATCCCAGGCTTCTGCTATCT	TTGAAAGGTACGTATCGCGG	50.0	50.0	57.9	58.1	921-930 bp	1.0	82.9
ycf1_p3	ACGTCTTTGAGAGCGCATAG	TGAGACAGGCGAAAGTGATG	50.0	50.0	58.1	57.9	964-973 bp	0.875	77.5
ycf1_p4	ATCCCAGGCTTCTGCTATCT	TGAGACAGGCGAAAGTGATG	50.0	50.0	57.9	57.9	1228-1237 bp	0.875	77.2
ycf1_p5	TTTACGTCTTTGAGAGCGCA	TGAGACAGGCGAAAGTGATG	45.0	50.0	57.9	57.9	967-976 bp	0.875	77.1