Hotspot

rpl32-trnL-UAG

SSC · 114632-115481 · 850 bp

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Pi 0.0196

MarkerSeek score 65.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 850
variable_sites: 47
pi: 0.0196
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGAAATCTCTTTCTACCGGG	TCGAACCGAGATGCTTTAGC	52.4	50.0	57.8	58.1	975-1028 bp	1.0	76.5
rpl32-trnL-UAG_p2	GGGAAATCTCTTTCTACCGGG	TCCTAAGAGCAGCGTGTCTA	52.4	50.0	57.8	57.9	949-1002 bp	1.0	76.3
rpl32-trnL-UAG_p3	GGGAAATCTCTTTCTACCGGG	AGCAGCGTGTCTACCAATTT	52.4	45.0	57.8	57.8	942-995 bp	1.0	75.9
rpl32-trnL-UAG_p4	GGGAAATCTCTTTCTACCGGG	CCTAAGAGCAGCGTGTCTAC	52.4	55.0	57.8	57.8	948-1001 bp	1.0	75.7
rpl32-trnL-UAG_p5	GGGAAATCTCTTTCTACCGGG	CTAAGAGCAGCGTGTCTACC	52.4	55.0	57.8	57.8	947-1000 bp	1.0	75.7