Hotspot

petA-psbJ

LSC · 64668-65669 · 1002 bp

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Pi 0.0187

MarkerSeek score 60.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1002
variable_sites: 46
pi: 0.0187
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GAGAAGGTTCAATTGGCCGA	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1043-1061 bp	1.0	80.8
petA-psbJ_p2	TCGAGAAGGTTCAATTGGCC	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1045-1063 bp	1.0	80.8
petA-psbJ_p3	AGAAGGTTCAATTGGCCGAA	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.7	57.6	1042-1060 bp	1.0	80.0
petA-psbJ_p4	GGTTCAATTGGCCGAAATGA	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.0	57.6	1038-1056 bp	1.0	74.0
petA-psbJ_p5	GAGAAGGTTCAATTGGCCGA	TGGTTCATATTCCGGATTGGG	50.0	47.6	58.2	57.8	1051-1069 bp	1.0	73.9