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atpH-atpI

LSC · 13908-14922 · 1015 bp

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Pi 0.0154

MarkerSeek score 56.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1015
variable_sites: 42
pi: 0.0154
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	1103-1123 bp	1.0	78.6
atpH-atpI_p2	AATAACGGAAGCGGCAGAAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.2	58.5	1104-1124 bp	1.0	77.4
atpH-atpI_p3	GCCAATCCAGCAGCAATAAC	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.8	58.5	1118-1138 bp	1.0	77.1
atpH-atpI_p4	ATAACGGAAGCGGCAGAAAT	CCGCGGCTTATATAGGTGAA	45.0	50.0	58.0	57.3	1088-1108 bp	1.0	76.9
atpH-atpI_p5	TCCAGCAGCAATAACGGAAG	TTTTTGCAACTTTAGCCGCG	50.0	45.0	58.3	58.5	1113-1133 bp	1.0	76.8