Hotspot

psbA-trnK-UUU

Genome · 55165-55409 · 245 bp

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Pi 0.0286
MarkerSeek score 54.30
Primer available yes
Label psbA-trnK-UUU

Feature Metadata

label_name
psbA-trnK-UUU
feature_type
igs
region
Genome
length_bp
245
variable_sites
7
pi
0.0286
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-trnK-UUU_p1 TAAAGGCGGTTTTCAGTGCT CACGGTTTGGGAAGAGATGT 45.0 50.0 58.0 57.8 431-433 bp 1.0 79.8
psbA-trnK-UUU_p2 GTAAAGGCGGTTTTCAGTGC CACGGTTTGGGAAGAGATGT 50.0 50.0 58.0 57.8 432-434 bp 1.0 79.6
psbA-trnK-UUU_p3 AAGCAGCTTCCCAAATTGGA CACGGTTTGGGAAGAGATGT 45.0 50.0 58.0 57.8 646-648 bp 1.0 79.4
psbA-trnK-UUU_p4 CATTCACGACCCATGTAGCA CACGGTTTGGGAAGAGATGT 50.0 50.0 58.0 57.8 737-739 bp 1.0 79.2
psbA-trnK-UUU_p5 CCGCAACAGGAGCTGAATAT CACGGTTTGGGAAGAGATGT 50.0 50.0 58.0 57.8 802-804 bp 1.0 79.0