Hotspot

trnS-UGA-psbC

Genome · 42572-42665 · 94 bp

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Pi 0.0638
MarkerSeek score 78.90
Primer available yes
Label trnS-UGA-psbC

Feature Metadata

label_name
trnS-UGA-psbC
feature_type
igs
region
Genome
length_bp
94
variable_sites
6
pi
0.0638
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-UGA-psbC_p1 GGGTTCGAATCCCTCTCTCT GATCGTGATTTCGAGCCTGT 55.0 50.0 58.2 58.1 162 bp 1.0 80.7
trnS-UGA-psbC_p2 GATGGCTCCGGTCTTGAAAA GATCGTGATTTCGAGCCTGT 50.0 50.0 58.2 58.1 207 bp 1.0 80.3
trnS-UGA-psbC_p3 GATGGCTCCGGTCTTGAAAA TGATCGTGATTTCGAGCCTG 50.0 50.0 58.2 58.1 208 bp 1.0 80.3
trnS-UGA-psbC_p4 GATGGCTCCGGTCTTGAAAA CGAGCCTGTTCTTTCCATGA 50.0 50.0 58.2 57.9 196 bp 1.0 80.2
trnS-UGA-psbC_p5 GATGGCTCCGGTCTTGAAAA TCGAGCCTGTTCTTTCCATG 50.0 50.0 58.2 57.9 197 bp 1.0 80.2