Hotspot

psbZ-trnS-UGA

Genome · 42270-42483 · 214 bp

Back to Ulota

Pi 0.00467
MarkerSeek score 49.90
Primer available yes
Label psbZ-trnS-UGA

Feature Metadata

label_name
psbZ-trnS-UGA
feature_type
igs
region
Genome
length_bp
214
variable_sites
1
pi
0.00467
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnS-UGA_p1 CAACCATCAGGAGAGGCAAG TTTTCAAGACCGGAGCCATC 55.0 50.0 58.3 58.2 352 bp 1.0 76.8
psbZ-trnS-UGA_p2 GCACGACAGGTACACCAATT TTTTCAAGACCGGAGCCATC 50.0 50.0 58.5 58.2 333 bp 1.0 75.0
psbZ-trnS-UGA_p3 CAACCATCAGGAGAGGCAAG GTTTTCAAGACCGGAGCCAT 55.0 50.0 58.3 58.5 353 bp 1.0 74.5
psbZ-trnS-UGA_p4 TGACCAACCATCAGGAGAGG TTTTCAAGACCGGAGCCATC 55.0 50.0 58.7 58.2 356 bp 1.0 73.0
psbZ-trnS-UGA_p5 GCACGACAGGTACACCAATT GTTTTCAAGACCGGAGCCAT 50.0 50.0 58.5 58.5 334 bp 1.0 72.7