Hotspot

trnT-UGU-rps4

Genome · 33095-33392 · 298 bp

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Pi 0.0201
MarkerSeek score 64.10
Primer available yes
Label trnT-UGU-rps4

Feature Metadata

label_name
trnT-UGU-rps4
feature_type
igs
region
Genome
length_bp
298
variable_sites
6
pi
0.0201
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-rps4_p1 GATGGTCATCGGTTCGACTC GAGGTCCTCGATAACGGGA 55.0 57.9 58.2 57.9 352-353 bp 1.0 71.2
trnT-UGU-rps4_p2 TGCTTAGCTCAGAGGTCAGA GAGGTCCTCGATAACGGGA 50.0 57.9 57.8 57.9 390-391 bp 1.0 70.6
trnT-UGU-rps4_p3 GCACTTGTAATGCGATGGTC GAGGTCCTCGATAACGGGA 50.0 57.9 57.5 57.9 365-366 bp 1.0 68.8
trnT-UGU-rps4_p4 AGAGCACCGCACTTGTAATG GAGGTCCTCGATAACGGGA 50.0 57.9 58.6 57.9 373-374 bp 1.0 68.0
trnT-UGU-rps4_p5 CAGAGCACCGCACTTGTAAT GAGGTCCTCGATAACGGGA 50.0 57.9 58.6 57.9 374-375 bp 1.0 68.0