Hotspot

rpl2-rps19

Genome · 2081-2135 · 55 bp

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Pi 0.0182
MarkerSeek score 63.00
Primer available yes
Label rpl2-rps19

Feature Metadata

label_name
rpl2-rps19
feature_type
igs
region
Genome
length_bp
55
variable_sites
1
pi
0.0182
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl2-rps19_p1 ATGGGGTCATACTGCACTTG GTCAGCTACAAAAGGGCCTT 50.0 50.0 57.6 58.1 182 bp 1.0 76.3
rpl2-rps19_p2 CATGGGGTCATACTGCACTT GTCAGCTACAAAAGGGCCTT 50.0 50.0 57.6 58.1 183 bp 1.0 76.3
rpl2-rps19_p3 GGGTCATACTGCACTTGGAA GTCAGCTACAAAAGGGCCTT 50.0 50.0 57.5 58.1 179 bp 1.0 75.8
rpl2-rps19_p4 ATGGGGTCATACTGCACTTG ATGGTCAGCTACAAAAGGGC 50.0 50.0 57.6 58.2 185 bp 1.0 75.7
rpl2-rps19_p5 CATGGGGTCATACTGCACTT ATGGTCAGCTACAAAAGGGC 50.0 50.0 57.6 58.2 186 bp 1.0 75.7