Hotspot

syfB

SSC · 96104-98224 · 2121 bp

Back to Ulnaria

Pi 0.122

MarkerSeek score 77.60

Primer available yes

Label syfB

Feature Metadata

label_name: syfB
feature_type: gene
region: SSC
length_bp: 2121
variable_sites: 259
pi: 0.122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
syfB_p1	CCTGCCACCCCAGTTAAAAT	TCACAACCCACTGCCTTAAC	50.0	50.0	58.1	58.0	2959-2973 bp	1.0	87.7
syfB_p2	CTCGTAGGTTCGAATCCTGC	TCACAACCCACTGCCTTAAC	55.0	50.0	58.2	58.0	2974-2988 bp	1.0	87.0
syfB_p3	CCACTCGTAGGTTCGAATCC	TCACAACCCACTGCCTTAAC	55.0	50.0	57.8	58.0	2977-2991 bp	1.0	86.6
syfB_p4	ACAGGTACAGCAACATTCCG	TCACAACCCACTGCCTTAAC	50.0	50.0	58.2	58.0	2769-2778 bp	1.0	86.5
syfB_p5	CCTGCCACCCCAGTTAAAAT	GCCTTAACCACTTGGCTACA	50.0	50.0	58.1	57.8	2947-2961 bp	1.0	86.4