Hotspot

rpoC2

LSC · 42348-46685 · 4338 bp

Back to Ulnaria

Pi 0.0877

MarkerSeek score 72.90

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4338
variable_sites: 380
pi: 0.0877
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TGCAACTAAAACAGGAGGGAC	TGCGTTTCTTCTTCCAACCA	47.6	45.0	58.1	58.0	258 bp	0.5	50.5
rpoC2_p2	CCAGATTCTTTTTCCCAGCCT	TGCGTTTCTTCTTCCAACCA	47.6	45.0	58.2	58.0	848 bp	0.5	50.1
rpoC2_p3	TCCAGATTCTTTTTCCCAGCC	TGCGTTTCTTCTTCCAACCA	47.6	45.0	58.2	58.0	849 bp	0.5	50.1
rpoC2_p4	CCAGATTCTTTTTCCCAGCC	TGCGTTTCTTCTTCCAACCA	50.0	45.0	56.7	58.0	848 bp	0.5	49.0
rpoC2_p5	TGCAACTAAAACAGGAGGGA	TGCGTTTCTTCTTCCAACCA	45.0	45.0	56.7	58.0	258 bp	0.5	48.9