Hotspot

petA

LSC · 30440-31384 · 945 bp

Back to Ulnaria

Pi 0.055

MarkerSeek score 78.80

Primer available yes

Label petA

Feature Metadata

label_name: petA
feature_type: gene
region: LSC
length_bp: 945
variable_sites: 52
pi: 0.055
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA_p1	AGTACACCAAGAAGACGAACT	AAGCATCCATCTTTTAAAAAGT	42.9	27.3	56.6	52.5	1052 bp	1.0	44.0
petA_p2	AGTACACCAAGAAGACGAACT	AAAGCATCCATCTTTTAAAAAGT	42.9	26.1	56.6	53.4	1053 bp	1.0	44.0
petA_p3	AGTACACCAAGAAGACGAACT	AGCATCCATCTTTTAAAAAGTATTTCC	42.9	29.6	56.6	57.1	1051 bp	1.0	44.0
petA_p4	AGTACACCAAGAAGACGAACT	GCATCCATCTTTTAAAAAGTATTTCCT	42.9	29.6	56.6	57.1	1050 bp	1.0	44.0
petA_p5	AGTACACCAAGAAGACGAACT	ACTAAAGCATCCATCTTTTAAAAAGT	42.9	26.9	56.6	56.1	1056 bp	1.0	43.9