Hotspot

rpl14-rps17

SSC · 85433-85442 · 10 bp

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Pi 0.1
MarkerSeek score 79.60
Primer available yes
Label rpl14-rps17

Feature Metadata

label_name
rpl14-rps17
feature_type
igs
region
SSC
length_bp
10
variable_sites
1
pi
0.1
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14-rps17_p1 GCACCAGTATTATCTGCTACTGT AGTTCAAGAATGTCGTCCTT 43.5 40.0 58.0 54.6 139 bp 1.0 40.6
rpl14-rps17_p2 AGCACCAGTATTATCTGCTACTG AGTTCAAGAATGTCGTCCTT 43.5 40.0 57.8 54.6 140 bp 1.0 40.6
rpl14-rps17_p3 GCACCAGTATTATCTGCTACTGT AGTTCAAGAATGTCGTCCTTT 43.5 38.1 58.0 55.5 139 bp 1.0 40.6
rpl14-rps17_p4 AGCACCAGTATTATCTGCTACTG AGTTCAAGAATGTCGTCCTTT 43.5 38.1 57.8 55.5 140 bp 1.0 40.6
rpl14-rps17_p5 ACCACCTAGAACCCTAATACACA AGTTCAAGAATGTCGTCCTT 43.5 40.0 58.3 54.6 173 bp 1.0 40.5