Hotspot

ycf45-trnP(TGG)

IRb · 61749-61978 · 230 bp

Back to Ulnaria

Pi 0.147
MarkerSeek score 78.80
Primer available yes
Label ycf45-trnP(TGG)

Feature Metadata

label_name
ycf45-trnP(TGG)
feature_type
igs
region
IRb
length_bp
230
variable_sites
31
pi
0.147
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf45-trnP(TGG)_p1 TGTAACGCTGAGTGATGACG GATTTGAACCTACGACCCCC 50.0 55.0 58.0 58.0 878 bp 0.5 59.5
ycf45-trnP(TGG)_p2 TGTAACGCTGAGTGATGACG GGATTTGAACCTACGACCCC 50.0 55.0 58.0 58.0 879 bp 0.5 59.5
ycf45-trnP(TGG)_p3 TGTAACGCTGAGTGATGACG AAACGACTTCTCGGCAAACT 50.0 45.0 58.0 58.1 1414 bp 0.5 59.4
ycf45-trnP(TGG)_p4 TGTAACGCTGAGTGATGACG AACGACTTCTCGGCAAACTT 50.0 45.0 58.0 58.1 1413 bp 0.5 59.4
ycf45-trnP(TGG)_p5 TGTAACGCTGAGTGATGACG GACTTCTCGGCAAACTTCCA 50.0 50.0 58.0 58.1 1410 bp 0.5 58.8