Hotspot

trnM(CAT)-trnS(GCT)

LSC · 17102-17191 · 90 bp

Back to Ulnaria

Pi 0.224
MarkerSeek score 80.40
Primer available yes
Label trnM(CAT)-trnS(GCT)

Feature Metadata

label_name
trnM(CAT)-trnS(GCT)
feature_type
igs
region
LSC
length_bp
90
variable_sites
17
pi
0.224
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.84
markerseek_score
80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM(CAT)-trnS(GCT)_p1 AGTGGTTAGAGCAGGGGATT CTTTCGACCACTCAGCCATT 50.0 50.0 58.0 58.2 161-175 bp 1.0 90.3
trnM(CAT)-trnS(GCT)_p2 AGTGGTTAGAGCAGGGGATT TTCGACCACTCAGCCATTTC 50.0 50.0 58.0 58.2 159-173 bp 1.0 90.2
trnM(CAT)-trnS(GCT)_p3 TGGTTAGAGCAGGGGATTCA CTTTCGACCACTCAGCCATT 50.0 50.0 58.0 58.2 159-173 bp 1.0 90.2
trnM(CAT)-trnS(GCT)_p4 TGGTTAGAGCAGGGGATTCA TTCGACCACTCAGCCATTTC 50.0 50.0 58.0 58.2 157-171 bp 1.0 90.2
trnM(CAT)-trnS(GCT)_p5 AGTGGTTAGAGCAGGGGATT GACCACTCAGCCATTTCTCC 50.0 55.0 58.0 58.3 156-170 bp 1.0 89.7