Hotspot

trnV(TAC)-trnY(GTA)

LSC · 12871-13050 · 180 bp

Back to Ulnaria

Pi 0.186

MarkerSeek score 82.40

Primer available yes

Label trnV(TAC)-trnY(GTA)

Feature Metadata

label_name: trnV(TAC)-trnY(GTA)
feature_type: igs
region: LSC
length_bp: 180
variable_sites: 32
pi: 0.186
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV(TAC)-trnY(GTA)_p1	TACCGCTGAGTTAATTGCCC	GATTTACAATCCGCCCCCTT	50.0	50.0	58.0	57.9	234-241 bp	1.0	89.9
trnV(TAC)-trnY(GTA)_p2	AGACACTCTACCGCTGAGTT	GATTTACAATCCGCCCCCTT	50.0	50.0	58.1	57.9	242-249 bp	1.0	89.4
trnV(TAC)-trnY(GTA)_p3	CTGTGGCCTTCTGCTTGTAA	GATTTACAATCCGCCCCCTT	50.0	50.0	58.1	57.9	265-272 bp	1.0	89.0
trnV(TAC)-trnY(GTA)_p4	TGTGGCCTTCTGCTTGTAAG	GATTTACAATCCGCCCCCTT	50.0	50.0	58.1	57.9	264-271 bp	1.0	89.0
trnV(TAC)-trnY(GTA)_p5	TACCGCTGAGTTAATTGCCC	CGTAACTTGACGTAGCGGAT	50.0	50.0	58.0	57.8	251-258 bp	1.0	88.8