Hotspot

trnK(TTT)-trnR(TCT)

LSC · 12651-12719 · 69 bp

Back to Ulnaria

Pi 0.364

MarkerSeek score 78.40

Primer available yes

Label trnK(TTT)-trnR(TCT)

Feature Metadata

label_name: trnK(TTT)-trnR(TCT)
feature_type: igs
region: LSC
length_bp: 69
variable_sites: 20
pi: 0.364
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK(TTT)-trnR(TCT)_p1	TAGTTCTGGGTTCGAGTCCC	TTCGATTCCTACTGGGCCTA	55.0	50.0	58.4	57.5	105-119 bp	1.0	84.7
trnK(TTT)-trnR(TCT)_p2	ACCGAATAGTTCTGGGTTCG	TTCGATTCCTACTGGGCCTA	50.0	50.0	57.3	57.5	111-125 bp	1.0	82.9
trnK(TTT)-trnR(TCT)_p3	TAGTTCTGGGTTCGAGTCCC	GGGCCTATCGTCTAACGGAT	55.0	55.0	58.4	58.7	158-172 bp	1.0	82.5
trnK(TTT)-trnR(TCT)_p4	ACCGAATAGTTCTGGGTTCG	GGGCCTATCGTCTAACGGAT	50.0	55.0	57.3	58.7	164-178 bp	1.0	80.7
trnK(TTT)-trnR(TCT)_p5	CCGAATAGTTCTGGGTTCGA	TTCGATTCCTACTGGGCCTA	50.0	50.0	57.0	57.5	110-124 bp	1.0	80.7