Hotspot

rpl32-trnL-UAG

SSC · 117574-118672 · 1099 bp

Back to Ulmus

Pi 0.0342
MarkerSeek score 74.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1099
variable_sites
166
pi
0.0342
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.95
markerseek_score
74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGGGAAATCTCTTTCTACGAAGA AGCGTGTCTACCGATTTCAC 39.1 50.0 56.7 58.0 1169-1421 bp 1.0 57.9
rpl32-trnL-UAG_p2 AGGGAAATCTCTTTCTACGAAGA CAGCGTGTCTACCGATTTCA 39.1 50.0 56.7 58.0 1170-1422 bp 1.0 57.8
rpl32-trnL-UAG_p3 AGGGAAATCTCTTTCTACGAAGA TCGAACCGAGATGCTTTAGC 39.1 50.0 56.7 58.1 1205-1457 bp 1.0 57.3
rpl32-trnL-UAG_p4 AGGGAAATCTCTTTCTACGAAGA TCCTAAGAGCAGCGTGTCTA 39.1 50.0 56.7 57.9 1179-1431 bp 1.0 56.9
rpl32-trnL-UAG_p5 GCATTGAAAGCTTTTTCATTAGGG AGCGTGTCTACCGATTTCAC 37.5 50.0 57.6 58.0 1189-1441 bp 0.913043 51.3