Hotspot

ndhF-rpl32

SSC · 116289-117414 · 1126 bp

Back to Ulmus

Pi 0.0275
MarkerSeek score 66.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1126
variable_sites
105
pi
0.0275
unique_haplotype_count
19
primer_available
yes
alignment_reliability
0.94
markerseek_score
66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CCCCACGCGATTAGTTACAA TGCTGCCCAATATCCTTTCC 50.0 50.0 58.0 57.9 1615-1795 bp 1.0 88.7
ndhF-rpl32_p2 TTTTTGACAAGCATTCGCCG TGCTGCCCAATATCCTTTCC 45.0 50.0 58.2 57.9 1591-1771 bp 1.0 87.0
ndhF-rpl32_p3 GATTTAGCAACTGCACCAGC TGCTGCCCAATATCCTTTCC 50.0 50.0 57.7 57.9 1782-1962 bp 1.0 86.5
ndhF-rpl32_p4 CCCCACGCGATTAGTTACAA GAAATCGTGTGCTTGCGTTT 50.0 45.0 58.0 58.2 2036-2228 bp 0.782609 79.2
ndhF-rpl32_p5 CCCCACGCGATTAGTTACAA AAAAGCTTTCAATGCTGCCC 50.0 45.0 58.0 57.8 1627-1777 bp 0.347826 60.1