Hotspot

trnR-UCU-atpA

LSC · 11240-11582 · 343 bp

Back to Ulmus

Pi 0.0222
MarkerSeek score 62.90
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
343
variable_sites
29
pi
0.0222
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.88
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 ATGGAAATGAAAGGCGTCCA TCAGGAACAGATGGAACGTT 45.0 45.0 57.8 56.8 433-483 bp 1.0 77.9
trnR-UCU-atpA_p2 ATGGAAATGAAAGGCGTCCA TTCAGGAACAGATGGAACGT 45.0 45.0 57.8 56.8 434-484 bp 1.0 77.9
trnR-UCU-atpA_p3 TGGAAATGAAAGGCGTCCAT TCAGGAACAGATGGAACGTT 45.0 45.0 57.8 56.8 432-482 bp 1.0 77.9
trnR-UCU-atpA_p4 TGGAAATGAAAGGCGTCCAT TTCAGGAACAGATGGAACGT 45.0 45.0 57.8 56.8 433-483 bp 1.0 77.9
trnR-UCU-atpA_p5 TGGAAATGAAAGGCGTCCAT TCAGGAACAGATGGAACGTTT 45.0 42.9 57.8 57.5 432-482 bp 1.0 75.8