Hotspot

rps16-trnQ-UUG

LSC · 6532-7749 · 1218 bp

Back to Ulmus

Pi 0.0342
MarkerSeek score 69.60
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1218
variable_sites
125
pi
0.0342
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.83
markerseek_score
69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1078-1291 bp 1.0 90.3
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA TAAGGCATCGGGTTTTGGTC 45.0 50.0 58.1 58.2 1108-1321 bp 1.0 89.5
rps16-trnQ-UUG_p3 GGATCGTGTCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 1092-1305 bp 1.0 88.4
rps16-trnQ-UUG_p4 GGATCGTGTCCTTCAAGTCG TAAGGCATCGGGTTTTGGTC 55.0 50.0 58.4 58.2 1122-1335 bp 1.0 87.7
rps16-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA GTAAGGCATCGGGTTTTGGT 45.0 50.0 58.1 58.5 1109-1322 bp 1.0 87.2