Hotspot

rpl16

LSC · 87725-89185 · 1461 bp

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Pi 0.0205
MarkerSeek score 72.10
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1461
variable_sites
29
pi
0.0205
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 CTGCTCTTTCAATGCAAAGGA TCCTCCTGTTTGTTGAATCA 42.9 40.0 57.1 54.5 1666-1667 bp 1.0 43.6
rpl16_p2 TCTGCTCTTTCAATGCAAAGG TCCTCCTGTTTGTTGAATCA 42.9 40.0 57.1 54.5 1667-1668 bp 1.0 43.6
rpl16_p3 CTGCTCTTTCAATGCAAAGGA TCCTCCTGTTTGTTGAATCAA 42.9 38.1 57.1 55.3 1666-1667 bp 1.0 43.6
rpl16_p4 CTGCTCTTTCAATGCAAAGGA TTCCTCCTGTTTGTTGAATCA 42.9 38.1 57.1 55.3 1667-1668 bp 1.0 43.6
rpl16_p5 TCTGCTCTTTCAATGCAAAGG TCCTCCTGTTTGTTGAATCAA 42.9 38.1 57.1 55.3 1667-1668 bp 1.0 43.6