Hotspot

ndhF-rpl32

SSC · 124834-126183 · 1350 bp

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Pi 0.0391
MarkerSeek score 80.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1350
variable_sites
51
pi
0.0391
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ACAGGGAGTGGAAGAAAAGG CGCAGCCAAATATCCTTTTT 50.0 40.0 57.0 54.9 1459-1487 bp 1.0 52.9
ndhF-rpl32_p2 CAGGGAGTGGAAGAAAAGGT CGCAGCCAAATATCCTTTTT 50.0 40.0 57.0 54.9 1458-1486 bp 1.0 52.9
ndhF-rpl32_p3 AACAGGGAGTGGAAGAAAAGG CGCAGCCAAATATCCTTTTT 47.6 40.0 57.8 54.9 1460-1488 bp 1.0 50.8
ndhF-rpl32_p4 ACAGGGAGTGGAAGAAAAGGT CGCAGCCAAATATCCTTTTT 47.6 40.0 58.9 54.9 1459-1487 bp 1.0 45.7
ndhF-rpl32_p5 TGACATAGTAACAGGGAGTGGA CGCAGCCAAATATCCTTTTT 45.5 40.0 57.9 54.9 1469-1497 bp 1.0 45.3