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rpl33-rps18

LSC · 74112-74314 · 203 bp

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Pi 0.0547

MarkerSeek score 75.80

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 203
variable_sites: 11
pi: 0.0547
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	AACACAATACACCCAGTCGA	ATTCGATCCCCCGATCCAAT	45.0	50.0	56.8	58.6	372-382 bp	1.0	68.0
rpl33-rps18_p2	CAACACAATACACCCAGTCGA	ATTCGATCCCCCGATCCAAT	47.6	50.0	58.2	58.6	373-383 bp	1.0	67.7
rpl33-rps18_p3	TCAACACAATACACCCAGTCG	ATTCGATCCCCCGATCCAAT	47.6	50.0	58.2	58.6	374-384 bp	1.0	67.7
rpl33-rps18_p4	CAACACAATACACCCAGTCG	ATTCGATCCCCCGATCCAAT	50.0	50.0	56.7	58.6	373-383 bp	1.0	67.6
rpl33-rps18_p5	AACACAATACACCCAGTCGA	ATCAATTCGATCCCCCGATC	45.0	50.0	56.8	57.2	376-386 bp	1.0	66.9