Hotspot

trnP(UGG)-psaJ

LSC · 72910-73361 · 452 bp

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Pi 0.0304

MarkerSeek score 71.30

Primer available yes

Label trnP(UGG)-psaJ

Feature Metadata

label_name: trnP(UGG)-psaJ
feature_type: igs
region: LSC
length_bp: 452
variable_sites: 13
pi: 0.0304
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 71.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP(UGG)-psaJ_p1	ACCCGTGACATTTTGTACCC	GGCATCCGGGAATAAACGAT	50.0	50.0	58.1	58.1	603-621 bp	1.0	83.7
trnP(UGG)-psaJ_p2	CCCGTGACATTTTGTACCCA	GGCATCCGGGAATAAACGAT	50.0	50.0	58.1	58.1	602-620 bp	1.0	83.7
trnP(UGG)-psaJ_p3	ACAGGATTTGAACCCGTGAC	GGCATCCGGGAATAAACGAT	50.0	50.0	58.1	58.1	614-632 bp	1.0	83.5
trnP(UGG)-psaJ_p4	CAGGATTTGAACCCGTGACA	GGCATCCGGGAATAAACGAT	50.0	50.0	58.1	58.1	613-631 bp	1.0	83.5
trnP(UGG)-psaJ_p5	TGACAGGATTTGAACCCGTG	GGCATCCGGGAATAAACGAT	50.0	50.0	58.1	58.1	616-634 bp	1.0	83.5