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rps4-trnT(UGU)

LSC · 49721-50267 · 547 bp

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Pi 0.0498

MarkerSeek score 79.70

Primer available yes

Label rps4-trnT(UGU)

Feature Metadata

label_name: rps4-trnT(UGU)
feature_type: igs
region: LSC
length_bp: 547
variable_sites: 24
pi: 0.0498
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT(UGU)_p1	CTAGTCAGTCCTGGCAAAGC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.3	57.9	622-678 bp	1.0	83.7
rps4-trnT(UGU)_p2	CTAGTCAGTCCTGGCAAAGC	GATGGTCATCGGTTCGACTC	55.0	55.0	58.3	58.2	591-647 bp	1.0	83.7
rps4-trnT(UGU)_p3	CTAGTCAGTCCTGGCAAAGC	CCGCTTAGCTCAGAGGTTAG	55.0	55.0	58.3	57.5	630-686 bp	1.0	80.8
rps4-trnT(UGU)_p4	CTAGTCAGTCCTGGCAAAGC	CAGAGGTTAGAGCATCGCAT	55.0	50.0	58.3	57.5	620-676 bp	1.0	80.6
rps4-trnT(UGU)_p5	CTAGTCAGTCCTGGCAAAGC	TCAGAGGTTAGAGCATCGCA	55.0	50.0	58.3	58.5	621-677 bp	1.0	80.6