Hotspot

trnE(UUC)-trnT(GGU)

LSC · 34243-35042 · 800 bp

Back to Typhonium

Pi 0.0112

MarkerSeek score 68.20

Primer available yes

Label trnE(UUC)-trnT(GGU)

Feature Metadata

label_name: trnE(UUC)-trnT(GGU)
feature_type: igs
region: LSC
length_bp: 800
variable_sites: 8
pi: 0.0112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE(UUC)-trnT(GGU)_p1	AGAGATGTCCTGAACCGCTA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	804-887 bp	1.0	83.5
trnE(UUC)-trnT(GGU)_p2	AGATGTCCTGAACCGCTAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.9	58.0	802-885 bp	1.0	83.5
trnE(UUC)-trnT(GGU)_p3	AGAGATGTCCTGAACCGCTA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	783-866 bp	1.0	83.1
trnE(UUC)-trnT(GGU)_p4	AGATGTCCTGAACCGCTAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.9	58.1	781-864 bp	1.0	83.1
trnE(UUC)-trnT(GGU)_p5	GATGTCCTGAACCGCTAGAC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	801-884 bp	1.0	83.0