Hotspot

ycf1

SSC · 130216-135732 · 5517 bp

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Pi 0.00672
MarkerSeek score 43.70
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5517
variable_sites
83
pi
0.00672
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 AAGGGGTCATACGTTTTGGG TGTCTTTGCATAGAACAGAA 50.0 35.0 57.8 52.4 223 bp 1.0 42.5
ycf1_p2 AAGGGGTCATACGTTTTGGG TGTCTTTGCATAGAACAGAAA 50.0 33.3 57.8 53.4 223 bp 1.0 42.5
ycf1_p3 AAGGGGTCATACGTTTTGGG TGTCTTTGCATAGAACAGAAAA 50.0 31.8 57.8 54.2 223 bp 1.0 42.5
ycf1_p4 AAGGGGTCATACGTTTTGGG ATGTCTTTGCATAGAACAGA 50.0 35.0 57.8 52.2 224 bp 1.0 42.5
ycf1_p5 AAGGGGTCATACGTTTTGGG ATGTCTTTGCATAGAACAGAA 50.0 33.3 57.8 53.1 224 bp 1.0 42.5