Hotspot

rpl16

LSC · 86369-87871 · 1503 bp

Back to Typha

Pi 0.0101
MarkerSeek score 57.90
Primer available yes
Label rpl16

Feature Metadata

label_name
rpl16
feature_type
gene
region
LSC
length_bp
1503
variable_sites
32
pi
0.0101
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16_p1 TCATCCTCAATACCCCTTTTTGT ACAAACTCCTTCATTCTTTTTCTGT 39.1 32.0 57.7 57.6 1627-1687 bp 1.0 43.4
rpl16_p2 TCATCCTCAATACCCCTTTTTGT TGACAAACTCCTTCATTCTTTTTCT 39.1 32.0 57.7 57.4 1629-1689 bp 1.0 43.4
rpl16_p3 TCATCCTCAATACCCCTTTTTGT TGACAAACTCCTTCATTCTT 39.1 35.0 57.7 52.3 1629-1689 bp 1.0 43.4
rpl16_p4 TCATCCTCAATACCCCTTTTTGT TGACAAACTCCTTCATTCTTT 39.1 33.3 57.7 53.3 1629-1689 bp 1.0 43.4
rpl16_p5 CATCCTCAATACCCCTTTTTGT ACAAACTCCTTCATTCTTTTTCTGT 40.9 32.0 56.3 57.6 1626-1686 bp 1.0 43.4