Hotspot

ccsA

SSC · 120204-121184 · 981 bp

Back to Typha

Pi 0.00632
MarkerSeek score 49.20
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
SSC
length_bp
981
variable_sites
12
pi
0.00632
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 GTGAAATCGGTAGACACGCT TTCTCGATGGCTTCTCTTGC 50.0 50.0 58.0 58.0 1723-1729 bp 1.0 81.4
ccsA_p2 TGAAATCGGTAGACACGCTG TTCTCGATGGCTTCTCTTGC 50.0 50.0 58.0 58.0 1722-1728 bp 1.0 81.4
ccsA_p3 GAGTGGCGGCATTCTCTAAA TTCTCGATGGCTTCTCTTGC 50.0 50.0 58.0 58.0 1663-1669 bp 1.0 81.4
ccsA_p4 GTGAAATCGGTAGACACGCT TGCATTACCCGGAATGAGTG 50.0 50.0 58.0 58.0 1706-1712 bp 1.0 81.3
ccsA_p5 TGAAATCGGTAGACACGCTG TGCATTACCCGGAATGAGTG 50.0 50.0 58.0 58.0 1705-1711 bp 1.0 81.3