Hotspot

psaC-ndhE

SSC · 123305-124022 · 718 bp

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Pi 0.0288
MarkerSeek score 76.60
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
718
variable_sites
41
pi
0.0288
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.97
markerseek_score
76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 ACATCCGTCCCAAGGTATCA TTATAGCCATTGCAGCTGCT 50.0 45.0 58.1 57.6 893-1020 bp 1.0 83.6
psaC-ndhE_p2 CCAATACATCTGTGGGGCAA TTATAGCCATTGCAGCTGCT 50.0 45.0 57.9 57.6 870-997 bp 1.0 83.5
psaC-ndhE_p3 ACATCCGTCCCAAGGTATCA TATAGCCATTGCAGCTGCTG 50.0 50.0 58.1 58.4 892-1019 bp 1.0 83.2
psaC-ndhE_p4 CCAATACATCTGTGGGGCAA TATAGCCATTGCAGCTGCTG 50.0 50.0 57.9 58.4 869-996 bp 1.0 83.2
psaC-ndhE_p5 AATACATCTGTGGGGCAAGC TTATAGCCATTGCAGCTGCT 50.0 45.0 58.2 57.6 868-995 bp 1.0 82.8