Hotspot

ccsA-ndhD

SSC · 121185-121433 · 249 bp

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Pi 0.026
MarkerSeek score 65.50
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
249
variable_sites
14
pi
0.026
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 CGGCACTTGTGGCTTCTATA ATCCCGATTTCGTTCTCTCA 50.0 45.0 57.7 56.4 431-437 bp 1.0 67.7
ccsA-ndhD_p2 CGGCACTTGTGGCTTCTATA TCCCGATTTCGTTCTCTCAT 50.0 45.0 57.7 56.4 430-436 bp 1.0 67.7
ccsA-ndhD_p3 CGGCACTTGTGGCTTCTATA TCCCGATTTCGTTCTCTCATT 50.0 42.9 57.7 57.1 430-436 bp 1.0 65.8
ccsA-ndhD_p4 CGGCACTTGTGGCTTCTATA ATCCCGATTTCGTTCTCTCAT 50.0 42.9 57.7 56.9 431-437 bp 1.0 64.0
ccsA-ndhD_p5 CGGCACTTGTGGCTTCTATA ATCCCGATTTCGTTCTCTCATT 50.0 40.9 57.7 57.6 431-437 bp 1.0 61.6