Hotspot

rpl22-rps19

IRb · 89105-89217 · 113 bp

Back to Typha

Pi 0.0265
MarkerSeek score 64.70
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
IRb
length_bp
113
variable_sites
7
pi
0.0265
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 CGCGCTTTGAAAACAGACAT TCTGACTTTCGCGAGACATG 45.0 50.0 58.0 58.0 239-285 bp 1.0 81.8
rpl22-rps19_p2 TTCGCGCTTTGAAAACAGAC TCTGACTTTCGCGAGACATG 45.0 50.0 57.9 58.0 241-287 bp 1.0 81.3
rpl22-rps19_p3 CGCGCTTTGAAAACAGACAT AATTCGCCCCTACTCTGACT 45.0 50.0 58.0 58.1 252-298 bp 1.0 80.8
rpl22-rps19_p4 CGCGCTTTGAAAACAGACAT ATTCGCCCCTACTCTGACTT 45.0 50.0 58.0 58.1 251-297 bp 1.0 80.8
rpl22-rps19_p5 CGCGCTTTGAAAACAGACAT AGAATTCGCCCCTACTCTGA 45.0 50.0 58.0 57.8 254-300 bp 1.0 80.5