Hotspot

rps8-rpl14

LSC · 85678-85866 · 189 bp

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Pi 0.0116
MarkerSeek score 66.70
Primer available yes
Label rps8-rpl14

Feature Metadata

label_name
rps8-rpl14
feature_type
igs
region
LSC
length_bp
189
variable_sites
5
pi
0.0116
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps8-rpl14_p1 AGATGCTATTCGAACCGTTCT GAACTCGAGTTTTTGGTGCG 42.9 50.0 57.2 58.0 342-370 bp 1.0 70.5
rps8-rpl14_p2 AGATGCTATTCGAACCGTTCT GCTCGGGAATTGAGACAGTT 42.9 50.0 57.2 57.9 319-347 bp 1.0 70.0
rps8-rpl14_p3 AGATGCTATTCGAACCGTTCT ACTCGAGTTTTTGGTGCGAT 42.9 45.0 57.2 58.1 340-368 bp 1.0 69.7
rps8-rpl14_p4 TGTAGATGCTATTCGAACCGT GAACTCGAGTTTTTGGTGCG 42.9 50.0 56.9 58.0 345-373 bp 1.0 68.4
rps8-rpl14_p5 AGATGCTATTCGAACCGTTCTT GAACTCGAGTTTTTGGTGCG 40.9 50.0 57.9 58.0 342-370 bp 1.0 68.0