Hotspot

ycf4-cemA

LSC · 64662-65528 · 867 bp

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Pi 0.00773
MarkerSeek score 65.50
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
867
variable_sites
14
pi
0.00773
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 ACAAAAAGCTGCCGAATTGG TGGGAAAGAAGGCAATGCTT 45.0 45.0 57.8 58.0 955-976 bp 1.0 80.7
ycf4-cemA_p2 TATTTCTTGCGCGTACCGAT TGGGAAAGAAGGCAATGCTT 45.0 45.0 57.8 58.0 933-954 bp 1.0 80.3
ycf4-cemA_p3 GCCATTCCCTTGACTCGTAC TGGGAAAGAAGGCAATGCTT 55.0 45.0 58.3 58.0 1005-1026 bp 1.0 79.1
ycf4-cemA_p4 ACAAAAAGCTGCCGAATTGG ATGGGAAAGAAGGCAATGCT 45.0 45.0 57.8 57.8 956-977 bp 1.0 78.9
ycf4-cemA_p5 TATTTCTTGCGCGTACCGAT ATGGGAAAGAAGGCAATGCT 45.0 45.0 57.8 57.8 934-955 bp 1.0 78.5