Hotspot

accD-psaI

LSC · 62494-63626 · 1133 bp

Back to Typha

Pi 0.0206
MarkerSeek score 64.50
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
1133
variable_sites
48
pi
0.0206
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 TATTTCAGCTCCACGGCTTC GAGGGTAAGTTGAGATCTGTCA 50.0 45.5 58.0 56.9 1210-1295 bp 1.0 61.2
accD-psaI_p2 TATTTCAGCTCCACGGCTTC AGGGTAAGTTGAGATCTGTCA 50.0 42.9 58.0 55.7 1209-1294 bp 1.0 59.5
accD-psaI_p3 AGTTATTTCAGCTCCACGGC GAGGGTAAGTTGAGATCTGTCA 50.0 45.5 58.3 56.9 1213-1298 bp 1.0 59.3
accD-psaI_p4 GTTATTTCAGCTCCACGGCT GAGGGTAAGTTGAGATCTGTCA 50.0 45.5 58.3 56.9 1212-1297 bp 1.0 59.3
accD-psaI_p5 CACGGCTTCTTTCCCTTGAA GAGGGTAAGTTGAGATCTGTCA 50.0 45.5 58.4 56.9 1199-1284 bp 1.0 58.3