Hotspot

ndhC-trnV-UAC

LSC · 53789-54879 · 1091 bp

Back to Typha

Pi 0.0121
MarkerSeek score 68.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1091
variable_sites
30
pi
0.0121
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTAACCGGGGCTAAAACTCC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.0 57.8 1228-1258 bp 1.0 80.5
ndhC-trnV-UAC_p2 TCTGGTCCTTCTCTAACCGG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.2 57.8 1240-1270 bp 1.0 79.5
ndhC-trnV-UAC_p3 CGGGGCTAAAACTCCTGAAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.8 57.8 1223-1253 bp 1.0 79.2
ndhC-trnV-UAC_p4 CTAACCGGGGCTAAAACTCC AGCCCAATGCAACAAAATGC 55.0 45.0 58.0 58.5 1310-1340 bp 1.0 78.1
ndhC-trnV-UAC_p5 TAACCGGGGCTAAAACTCCT AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.3 57.8 1227-1257 bp 1.0 78.0