Hotspot

psaA-ycf3

LSC · 44441-45093 · 653 bp

Back to Typha

Pi 0.00573
MarkerSeek score 65.60
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
653
variable_sites
7
pi
0.00573
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.97
markerseek_score
65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 TGAGAAATGACCGGGTTTGG TTGAAGATCACGAAGCGCTT 50.0 45.0 58.1 58.2 764-789 bp 1.0 80.5
psaA-ycf3_p2 TGAGAAATGACCGGGTTTGG GCTATAGCGCTTACTCCAGG 50.0 55.0 58.1 57.6 809-834 bp 1.0 79.1
psaA-ycf3_p3 TGAGAAATGACCGGGTTTGG CCGATCAAGCTGCTGAGTAT 50.0 50.0 58.1 57.5 838-863 bp 1.0 77.7
psaA-ycf3_p4 TGAGAAATGACCGGGTTTGG TCCGATCAAGCTGCTGAGTA 50.0 50.0 58.1 58.5 839-864 bp 1.0 77.7
psaA-ycf3_p5 TGAGAAATGACCGGGTTTGG TGGTTGAAGATCACGAAGCG 50.0 50.0 58.1 58.6 767-792 bp 1.0 77.5